Materials and Methods

Find the report of an unknown bacteria . ( all the lab tested was done and I have wrote the lab results for reference in the last page and have already found my unknown ) please help me writing good explanations in this report. I believe it is Proteus vulgaris

 

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BIOL 2420 UNKNOWN REPORT STUDENT ‘S NAME: ————————-

UNKNOWN NUMBER ——— GENUS AND SPECIES OF UNKNOWN: ————–

Objective:

 

 

Materials and Methods: Stains and biochemical tests were used to identify microorganism. Procedures from lab manual, and instructor’s power points were followed.

Stock cultures were kept of the organism. Periodic subculture was performed to keep the culture fresh.

 

TEST RESULTS: STAINS

**Source: specify the culture used to perform the stain: NA plate, or NB broth.

GRAM STAIN #1 Date: ——–

Source:

 

Result:

 

GRAM STAIN #2 Date: ——–

Source:

 

Result:

 

GRAM STAIN #3 Date: ——–

Source:

 

Result:

 

 

 

GRAM STAIN #4 Date: ——–

Source:

 

Result:

 

OTHER STAINS

ENDOSPORE STAIN Date: ——–

Source:

Result:

ACID FAST STAIN Date: ——–

Source:

Result:

TEST RESULTS GROWTH CHARACTERISTICS

OXYGEN CHARACTERISTICS: SOFT AGAR

DESCRIBE GROWTH:

 

INTERPRETATION/OXYGEN REQUIREMENT:

 

GROWTH TEMPERATURE

4C ———- 25C ———- 37C ———-

42C ——— 55C ———- 60C ———-

 

CONTROL OF GROWTH

Response to UV light:

Time of exposure: Results:

Time of exposure: Results:

Response to Temperature:

0C: ——– 60C: ——- 80C: ——–

100C: ——–

 

TEST RESULTS: BIOCHEMICAL TESTS

*”DATE “corresponds to inoculation date.

*Note incubation temperature other than 37C

 

GLUCOSE FERMENTATION:

DATE: INCUBATION TIME:

Description of Reaction:

 

Interpretation of Result:

 

LACTOSE FERMENTATION:

DATE: INCUBATION TIME:

Description of Reaction:

 

Interpretation of Result:

 

SUCROSE FERMENTATION:

DATE: INCUBATION TIME:

Description of Reaction:

 

Interpretation of Result:

 

MANNITOL FERMENTATION:

DATE: INCUBATION TIME:

Description of Reaction:

 

Interpretation of Result:

 

 

INDOLE PRODUCTION:

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

HYDROGEN SULFIDE PRODUCTION:

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

CITRATE UTILIZATION :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

MOTILITY :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

MR :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

VP :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

NITRATASE TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

UREASEASE TEST :

DATE: INCUBATION TIME:

Description of Reaction:

 

Interpretation of Result:

CATALASE TEST :

DATE:

Description of Reaction:

 

Interpretation of Result:

 

OXIDASE TEST :

DATE:

Description of Reaction:

 

Interpretation of Result:

 

LIPID HYDROLYSIS TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

STARCH HYDROLYSIS TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

BIL ESCULIN TEST:

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

OXYGEN REQUIREMENT :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

 

Interpretation of Result:

TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

TEST:

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

TEST:

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

TEST :

DATE: INCUBATION TIME

Description of Reaction:

 

Interpretation of Result:

 

CONCLUSSION

BACTERIA UNKNOWN GROUP:

Based on gram reaction results your bacteria unknown should be one of the following:

1.- Gram positive cocci, 2.- gram positive rod, or 3.- gram negative rod:

 

 

 

LIST ALL THE BACTERIA WITHIN YOUR UNKNOWN GROUP, AND SHOW HOW EACH OF THE ORGANISMS IN THE GROUP WERE ELIMINATED BASED ON THREE TEST RESULTS.:

Bacteria Elimination

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

 

Bacteria Elimination

 

 

 

 

 

Use this page to explain any problems you may have encountered while trying to identify your unknown. Give dates, and detailed description of the problems.

 

 

 

 

 

 

 

Once you have correctly identified your organism, think about what you would do differently if you had to do it again. Use this page to make a flow chart to outline a quick and efficient process to identify your organism. The chart should not include unnecessary tests. It should include all the tests needed to eliminate any other possible organism from the list of possible unknowns.

 

 

GRADING GRID

PROVIDES CORRECT UNKNOWN IDENTIFICATION AND NUMBER — 50 PTS.

PROVIDES A GRAM STAIN SLIDE , AND A RECENT NA STREAK PLATE OF UNKNOWN 10 PTS.

OBJECTIVES OUTLINED – 5 PTS.

ACURATE AND COMPLETE DESCRIPTION OF STAINS AND BIOCHEMICAL TESTS 10 PTS.

COMPLETED DESCRIPTION OF METHOD OF IDENTIFICATION, INCLUDING PROBLEMS ENCOUNTERED 10 PTS.

FLOW CHART FOR QUICK UNKNONW IDENTIFICATION 5 PTS.

UNKNOWN SUMMARY CHART 5 PTS.

NAME OF BACTERIA WRITTEN CORRECTLY ACCORDING TO RULES OF NOMENCLATURE/REPORT WRITTEN NEATLY AND LEGIBLY/ PAGES COMPLETED 5 PTS.

 

 

Notes:

 

 

 

 

(this two pictures are based on my lab report results that i got in lab for this report. Which shows that my unknown bacteria is proteus vulgaris.)

Also for the reference i got my SIM WHERE S= h2s production , I= indole production (+ve) and M= motility = +ve(POSITIVE)

Nitrate broth = red( present of no2)

 

 

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